Abstract

Arisarum vulgare, an alkaloid plant from the Araceae family native to the Mediterranean region,is used in traditional medicine for various ailments. This study aimed to evaluate the biological activities, par-ticularly anti-inflammatory, antioxidant, and antimicrobial properties, of ethanolic, methanolic, and aqueous extracts of this plant. Anti-inflammatory activity was assessed in vivo using an experimental asthma model with 33 rats divided into 6 groups, including controls and groups treated with plant extracts or prednisolone.
Inflammatory parameters, oxidative stress, and antioxidant enzyme activities were measured. Antioxidant activities were explored using DPPH, FRAP, and β-carotene bleaching methods. Results showed a significant reduction in inflammatory cells, especially eosinophils, in both blood and bronchoalveolar lavage fluid of rats treated with plant extracts. Oxidative stress decreased, with lower MDA levels and enhanced antioxidant enzyme activities. The LCMS-MS analysis of the ethanolic extract revealed a diverse chemical composition dominated by phenolic acids and flavonoids. Fumaric acid was the most abundant compound (37.24%), fol-
lowed by vanillic acid (24.69%) and 4-hydroxybenzoic acid (13.52%). Flavonoids such as kaempferol, luteolin, and myricetin were present in smaller quantities. The ethanolic extract demonstrated superior biochemical composition and antioxidant activity compared to the aqueous extract, with total phenol values of 74±5.11 mg GAE/mL versus 46 ± 0.66 mg GAE/mL, and DPPH IC50 values of 0.331 mg VS 2.095 mg, respectively. Antimi-crobial activity was observed only for ethanolic and methanolic extracts, with varying efficacy against tested
strains. The Ethanolic extract showed inhibition zones against Escherichia coli (12.33 ± 4.61 mm), Serratia sp. (14.33 ± 3.21 mm), Candida albicans (13.66±1.52 mm), and Fusarium roseum (10 ± 1.732 mm). The Meth-anolic extract was effective against Klebsiella pneumoniae (10.66 ± 4.04 mm), Staphylococcus aureus (11.66 ±0.57 mm), and Candida albicans (12.33 ± 1.52 mm). Arisarum vulgare shows promising therapeutic potential against various inflammatory, oxidative, and microbial diseases.

Information

Item Type:	Journal
Divisions:
ePrint ID:	5383
Date Deposited:	2025-05-05
Further Information:	Google Scholar
URI:	https://www.univ-soukahras.dz/en/publication/article/5383

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